Sporulation of Clostridium acetobutylicum P262 in a Defined Medium.
نویسندگان
چکیده
A defined minimal sporulation medium for Clostridium acetobutylicum P262, which produces high levels of solvents, is described. The overall sporulation sequence was similar to that of other endospore-forming bacteria. However, we observed a presporulation stage, during which swollen phase-bright cells which contained large amounts of granulose formed. During sporulation, the initiation of spore coat formation occurred before the onset of cortex formation. Other Clostridium strains tested showed marked variations in ability to grow and sporulate in various minimal media.
منابع مشابه
Transposon mutagenesis of Clostridium acetobutylicum P262: isolation and characterization of solvent deficient and metronidazole resistant mutants.
An efficient transposon mutagenesis system using conjugative transposons Tn916 and Tn925::Tn917 was established for Clostridium acetobutylicum P262, an industrial strain which has proved difficult to manipulate genetically. Transposon insertions occurred at several different locations to produce a variety of mutants. An oligosporogenous mutant deficient in acetone and butanol production, and tw...
متن کاملAutolytic Activity and an Autolysis-Deficient Mutant of Clostridium acetobutylicum.
The optimum conditions for autolysis and autoplast formation in Clostridium acetobutylicum P262 have been defined. Autolysis was optimal at pH 6.3 in 0.04 M sodium phosphate buffer, and the bacterium produced latent and active forms of an autolytic enzyme. The ability of cells to autolyze decreased sharply when cultures entered the stationary phase. Autoplasts were induced by 0.25 to 0.5 M sucr...
متن کاملCharacterization, Biosynthesis, and Regulation of Granulose in Clostridium acetobutylicum.
Synthesis of granulose was investigated in 15 solvent-producing Clostridium strains. Only one of the strains did not produce granulose. The structure of granulose in Clostridium acetobutylicum P262 consisted of a high-molecular-weight polyglucan containing only (1-->4) linked d-glucopyranose units. Biosynthesis of granulose in C. acetobutylicum P262 was dependent on ADPglucose pyrophosphorylase...
متن کاملIsolation and characterization of butanol-resistant mutants of Clostridium acetobutylicum.
In a wild-type strain of Clostridium acetobutylicum isolated from soil, solvent production appeared limited by butanol toxicity. Butanol-resistant mutants have been obtained which produced significantly higher solvent concentrations (about 30%) than the wild-type strain. Some other physiological differences were observed between a selected resistant mutant and the wild-type strain at the level ...
متن کاملGenomic-Based Identification of the Sporulation Restoring Gene in Degenerate Clostridium Acetobutylicum Strains
There is a renewed interest in the study of Clostridium acetobutylicum due to its applicability in renewable and “greener” production methods for alternative fuels and industrial solvents. Furthermore, due to significant advances in genetic technologies, C. acetobutylicum has ostensibly become a model clostridia for studying other solventogenic and pathogenic clostridia. Of considerable interes...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 45 4 شماره
صفحات -
تاریخ انتشار 1983